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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could add to tumorigenesis. This protein was identified to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but detailed nucleotide sequence facts just isn't yet offered.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized with the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This guideline outlines even more achievable optimization of mobile check here and immunoassay parameters to be sure the best possible success are...
Antibodies certain to the blocking peptide no longer bind to your epitope within the concentrate on protein. By comparing the staining within the blocked antibody compared to the antibody by itself, one can see which staining is precise.
Goal Information and facts Cyclin E belongs for the really conserved cyclin loved ones, whose users are characterized by a remarkable periodicity in protein abundance through the cell cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation patterns which add towards the temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells development by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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